A research assistant/ technician position is immediately available in Dr. Karen Bussard’s laboratory within the Department of Cancer Biology at Thomas Jefferson University, Sidney Kimmel Cancer Center, located in downtown Philadelphia. Dr. Bussard, an NIH funded investigator, is looking for a highly motivated individual with an interest in breast cancer bone biology and the tumor microenvironment. The laboratory utilizes murine models of metastasis, primary cell culture, molecular biology techniques, high-end fluorescence microscopy, 3-D models, exosomal analysis, and patient samples to answer questions related to disseminated breast tumor cells and breast cancer cell quiescence in the bone microenvironment.
Recent work in the laboratory has identified candidate cellular and exosomal microRNAs that impact breast cancer cell dormancy in the bone. We are interested not only in the way these factors impact the disseminated breast cancer cells, but also how the factors impact the surrounding bone stroma. The laboratory is also interested in identifying novel mediators of breast cancer cell metastasis to bone, with an emphasis on late-stage disease.
Candidates must have at least a baccalaureate degree in cell biology, molecular biology, or a closely related field, and at least four years laboratory experience working with sophisticated techniques including cell culture and molecular biology. Candidates will be working with cell-based models of cancer, and murine models of cancer metastasis. Must be proficient in mouse handling/ experimentation, models of cancer, and performing molecular biology techniques including cloning/ plasmid manipulation, transfection, knock-in/knock-out experiments, real-time PCR, RT-PCR, immunoblotting, ELISA, microarray analysis, IHC/IF, standard cell culture, and fluorescence & confocal microscopy. Experience with 3D models of cell culture, exosomes, microRNA, and/or a bioengineering background are desirable.
Candidates should have excellent oral and written communication skills, highly developed interpersonal skills, and display initiative as well as independence. Additional duties include overall lab maintenance, as well as the training of graduate students.
Jefferson Health delivers state-of-the-art healthcare services to patients throughout the Delaware Valley and southern New Jersey. Jefferson (Philadelphia University + Thomas Jefferson University) provides more than 7,800 students from nearly 40 states and 40 countries with 21 st century professional education. We have 30,000 employees.
Jefferson Health , with 14 hospitals (three are Magnet-designated by the ANCC for nursing excellence) and 50+ outpatient and urgent care locations, offers a broad range of primary and complex, highly specialized care that touches the lives of more than four million patients annually . This year, U.S. News & World Report has ranked Thomas Jefferson University Hospital as the 16 th best hospital in the nation, and among the nation’s best in 11 specialties. Jefferson Health also includes the NCI-designated Sidney Kimmel Cancer Center; it is one of only 69 such centers in the nation.
Thomas Jefferson University, nationally recognized for advancing groundbreaking developments in healthcare education and research, recently combined with Philadelphia University, internationally known for architecture, design, engineering, textiles and more. Jefferson (Philadelphia University + Thomas Jefferson University) has nine colleges and three schools dedicated to interprofessional and transdisciplinary approaches to learning that offer a vibrant and expandable platform for professional education. Through this unique model, we are preparing our students for current and yet-to-be-imagined careers.
As an employer, the Jefferson Enterprise maintains a commitment to provide equal access to employment. Jefferson values diversity and encourages applications from women, members of minority groups, LGBTQ individuals, disabled individuals, and veterans.